- First seen in Alison's funeral ("Pilot").
- Aria and Ezra share a second kiss in the church ("Pilot").
- Melissa leaves her phone here after meeting the minister to plan a christening for unborn Taylor Thomas ("For Whom the Bell Tolls").
- Ian tries to kill Spencer here ("For Whom the Bell Tolls").
- Alison (masked in black attire; her identity as Ian's attempted killer isn't revealed until "A is for Answers") attempts to kill Ian here by throwing him into the bell ropes in the tower to rescue Spencer ("For Whom the Bell Tolls").
- Ashley makes Hanna volunteer to sort clothes for a church rummage sale ("That Girl is Poison").
- Ted Wilson gives Ashley Marin the flashdrive that contains videos from the N.A.T. Club. ("Single Fright Female").
- Spencer has a flashback of a day when she, Alison and Mona were at church ("Will The Circle Be Unbroken?").
- Caleb's father Jamie Doyle is hired by Ted to repair the church bell tower ("Will The Circle Be Unbroken?").
- "A" steals the new church bell and replaces it with one of lesser value, in order to frame Jamie ("I'm Your Puppet").
- The church is located on Main Street.
- Though the same exterior seems to have been used, Tom and Isabel's wedding did not take place in the church in Rosewood.
- Ella Montgomery is on the church's Restoration Committee.
- The church is the site of Charlotte DiLaurentis' murder.